Insertion junction: CLIP2.042135_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042135
Chromosome:chromosome 1
Location:1817900
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010201 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTGGAGGTGAGGACTGTGTCGCAGTAGCCCCCAGCCGCAATGGTGACG
Internal bar code:CTTTGTAAAGAAGTCTGGAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:4378
LEAP-Seq percent confirming:85.9649
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACGTGCGCAGTTGTTGG
Suggested primer 2:GTAGCAAATGAAGTGGGCGG