| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042136 |
| Chromosome: | chromosome 6 |
| Location: | 1489611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260550 | CYG1 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGCCCGCCTCAATGCCAACCTCTGGCGGCATGCTGCTGTTGCCGTC |
| Internal bar code: | TTGTATCATAACTGGGTCACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 40.9091 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTCATCATTGCTCACCA |
| Suggested primer 2: | CTGTGACGGTTGCAAGGTTG |