| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042155 |
| Chromosome: | chromosome 13 |
| Location: | 5059213 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606600 | CGK1 | cGMP-dependent protein kinase; (1 of 6) K07376 - protein kinase, cGMP-dependent (PRKG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAACGGAGGAGCTCGACCCAGCGGAGCTTGCGGACGCGCCGCTGACG |
| Internal bar code: | TACGGGCCGTGCTGTGGATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4945 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCAGCGACAGGGTAAAAT |
| Suggested primer 2: | CTTCCTCAACCTGGTGACCC |