| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042165 |
| Chromosome: | chromosome 13 |
| Location: | 3694979 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588271 | (1 of 1) PF07556 - Protein of unknown function (DUF1538) (DUF1538) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGATGAGGATGGAATTCATACATGTTAAAATACGCGCTGCTGGGG |
| Internal bar code: | GGTCAGAGGGGGTTTTAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACTCCTTTCCCACGCTC |
| Suggested primer 2: | CACACGACCTACAGCTTGGT |