Insertion junction: CLIP2.042171_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042171
Chromosome:chromosome 10
Location:3425704
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g444100 (1 of 2) PF04564//PF05049 - U-box domain (U-box) // Interferon-inducible GTPase (IIGP) (IIGP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGTTCGGGCCTGCAATTCTAGATAGCTGGCGGCGCGCACACTGACTCG
Internal bar code:TCATATGTGACGTTACTAGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5896
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACCGGAACAGGTGGATA
Suggested primer 2:TCCTTGCTCGACAGGAAACC