Insertion junction: CLIP2.042186_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.042186
Chromosome:chromosome 6
Location:8009204
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g304750 (1 of 2) K12486 - stromal membrane-associated protein (SMAP) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATAGCGCGACGGACTTCACCTCGGCGCCACCCACCACAGCACTGCAC
Internal bar code:ATTGCGTGACGGTCTTAGCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1662
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACAACAAGTTGGGCACG
Suggested primer 2:CTGACAAGCTGCTGGACTCA