| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042239 |
| Chromosome: | chromosome 2 |
| Location: | 6462859 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGGACCGTGACCGCGAGCGCGTGCGCGTGGACCGGGATCCACTGCTC |
| Internal bar code: | GGAGCGGTATTCCCGGAGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3547 |
| LEAP-Seq percent confirming: | 56.25 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCCATTACAGCCAGGAC |
| Suggested primer 2: | TGTGTGCCACTGACAGACTC |