| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042262 |
| Chromosome: | chromosome 13 |
| Location: | 5026381 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606300 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGAGGGTACGGGTGCTGATGCTGCTGATGCTGATGCCGCCGACACA |
| Internal bar code: | AATCGGTTCGACCATTTTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1170 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCGAAAGACAAGCAC |
| Suggested primer 2: | GGAGTTGACACTGAGCGGAA |