| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042267 |
| Chromosome: | chromosome 17 |
| Location: | 3282142 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722050 | (1 of 1) K19202 - histone deacetylase complex subunit SAP30 (SAP30) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAAGGGAAAGGCGGAATTCTGTGTCATTGTTCATCGTCGAACGGCTG |
| Internal bar code: | CATAATGCTGCGGGCTTCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2850 |
| LEAP-Seq percent confirming: | 93.0435 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTGACACCGGTTGTTG |
| Suggested primer 2: | ACTGATGTGGCTGGTGAAGG |