Insertion junction: CLIP2.042279_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.042279
Chromosome:chromosome 16
Location:7059975
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g674739 (1 of 1) PF12537 - The Golgi pH Regulator (GPHR) Family N-terminal (GPHR_N) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGCAGGAAACTTTGAAACAGCTTCGTAGCAACTTCATTCTGTCCTGTG
Internal bar code:ATCATGGGTCAAAGGTCATTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:363
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTTGGGTGTCGTAGAAGA
Suggested primer 2:GCCCTAGATTCTCTGCCGAC