Insertion junction: CLIP2.042280_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.042280
Chromosome:chromosome 1
Location:111883
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g000750 ZFR2 zinc finger protein; (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGTAATTGCAGCTAAAAACTTCACCTGGCCCAGGCCTTTGCGAACAG
Internal bar code:TTGTCCCTATCTGTGCGCTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCGACTCATGTTCACAC
Suggested primer 2:GTAAAGAGGTCACCTGCCCC