| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042283 |
| Chromosome: | chromosome 13 |
| Location: | 3919533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589940 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTGGTAAGTGACGCCCGGAGAGAGCGTGGGTAGGACCAGACCAAAGT |
| Internal bar code: | AGGATCCCCCTAAGGTTCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2218 |
| LEAP-Seq percent confirming: | 46.9697 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTTTCCCAAGACGTCTC |
| Suggested primer 2: | TCGCAAAGTACGACTTGCCT |