| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042285 |
| Chromosome: | chromosome 4 |
| Location: | 451099 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217920 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCCCCAGGCGTGCATGTCACACCGGCTGGCCGTGGCAGCCGACGTG |
| Internal bar code: | GGGGCAGCGGTTTCTTAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3179 |
| LEAP-Seq percent confirming: | 79.4118 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCTGCTTGTGGTCCTG |
| Suggested primer 2: | AGGTGGGTAGCACGTGATTG |