| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042317 |
| Chromosome: | chromosome 12 |
| Location: | 5287360 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528100 | CPZ | Zinc carboxypeptidase; (1 of 1) PTHR12756:SF12 - CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCGGTAATACGCGTCCCTTGATCGGGCTCATGGAGTTATGGGGGAGC |
| Internal bar code: | TGTGCCGTAGCACGTATGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4574 |
| LEAP-Seq percent confirming: | 98.6014 |
| LEAP-Seq n confirming: | 141 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 143 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACGAAGCTTAGACTGGA |
| Suggested primer 2: | ACCACTCACTAAGCTGCACC |