Insertion junction: CLIP2.042321_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042321
Chromosome:chromosome 2
Location:5301053
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g112950 (1 of 1) PF09807 - Elongation complex protein 6 (ELP6) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTCCTGCTGTCGACAGGTGCCGTACTGCTTCGTGGCGGGTGCGTATGA
Internal bar code:GTCTGGCGGCGCTGCCGCAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3487
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AACGGCTTCACATTGTTGGC
Suggested primer 2:CTTGGATGCGAGGGTCAGTT