| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042326 |
| Chromosome: | chromosome 16 |
| Location: | 4525295 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801958 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCACGCACACACACGCCCCCGTGCAGCCCTGTGCCCGCACCGCCAGC |
| Internal bar code: | TTTTTGGTCATGTCATAAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCCCTCCATTGACGCTT |
| Suggested primer 2: | TCTCACACCCCCATCTCACT |