| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042342 |
| Chromosome: | chromosome 4 |
| Location: | 1592377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213250 | (1 of 2) K01256 - aminopeptidase N (pepN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGCTGGAGTCGCTGGTCAAGTCCATGAAGTGGGACGAGGTGGGGGA |
| Internal bar code: | TTGAGTGGCATGGTAGGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 2.85714 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCTCCCGTTTATGCCT |
| Suggested primer 2: | ACACACACAAGCACCTCACA |