| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042354 |
| Chromosome: | chromosome 16 |
| Location: | 2698343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661750 | (1 of 2) PF08332 - Calcium/calmodulin dependent protein kinase II association domain (CaMKII_AD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTAGCGGGCGCGGACCTTGCTGCGGGTGCCATCGTCGTTCTTCAGGG |
| Internal bar code: | CCGATAGACACACGCACTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2788 |
| LEAP-Seq percent confirming: | 72.4138 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTGCCTATCGTATGACA |
| Suggested primer 2: | CATTCACAGTCGCGCTAAGC |