| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042355 |
| Chromosome: | chromosome 2 |
| Location: | 1795333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800183 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCTTTAAGTCGGGCGACCCTACCGAGCCCTCTAATTATCGCCCCAT |
| Internal bar code: | GAGTATGATCTGTCGTATCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4327 |
| LEAP-Seq percent confirming: | 2.89855 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGACACCCACGTAAGAAA |
| Suggested primer 2: | TGATCCTGTTGAGCTGCTGG |