Insertion junction: CLIP2.042374_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042374
Chromosome:chromosome 2
Location:5883807
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g117410 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACGGCAGCCCTCGCTGCTACAGGCCCACACCACAGGCCTACACCACA
Internal bar code:TTGTCTTTAAGGGTTCGGGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1301
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACGAGAAGGAGGAGGT
Suggested primer 2:TCAAACACCAGGTTGCCCTT