Insertion junction: CLIP2.042393_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.042393
Chromosome:chromosome 16
Location:1515327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g653150 CPR3 (1 of 2) PF01841 - Transglutaminase-like superfamily (Transglut_core); Cysteine protease, transglutaminase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAGGCCTTGCCGGGCATCACGTGACGTCGGCGAAAACCCCAGCCCCTT
Internal bar code:CATATATTGGGTTTTGAAATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CATAATCTCGGGCGTCTGCT
Suggested primer 2:CCCTGTCCACTAAGGCCTTG