Insertion junction: CLIP2.042400_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042400
Chromosome:chromosome 16
Location:5855152
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g679700 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATATCGATCCCAAAGAGGTGGGGATGGACAGGGCGCAGCAACAGGGGG
Internal bar code:GGTTTATTATTTTGATCTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:252
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATCCCAACCTCAACCTC
Suggested primer 2:AGGGGAGGCTTCTCGAAGAA