| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042410 |
| Chromosome: | chromosome 4 |
| Location: | 3420127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227600 | (1 of 1) PTHR27000:SF77 - LEUCINE-RICH REPEAT PROTEIN KINASE-LIKE PROTEIN | 5'UTR | |
| Cre04.g800491 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAACGCTGACGACCCAGATGTTAGAACTGTGCAAATACCCTAGAAATT |
| Internal bar code: | GCGCGCCTCCCGAACAGTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2552 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGGCAGTCTCTTCGTGT |
| Suggested primer 2: | TGTAGCCCCTGACAGCATTG |