| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.042416 |
| Chromosome: | chromosome 12 |
| Location: | 7814977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553900 | CNG2 | (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATATGGGTGCCACTCGGGGTCAGGTCGTTCGTCGATCGCCCGAGGGG |
| Internal bar code: | GTGCTCAGTCACGCTGATAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 67.5 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATCAGCTTGTCGCCCAA |
| Suggested primer 2: | TTCGCTGCCTTCTAGTGCTC |