Insertion junction: CLIP2.042416_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042416
Chromosome:chromosome 12
Location:7814980
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553900 CNG2 (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTGTATGCGCCGCGCCCTGGGCGCGCTACAACGGTGACCCAGCACGC
Internal bar code:GTGCTCAGTCACGCTGATAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4608
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGCTGCCTTCTAGTGCTC
Suggested primer 2:ATGATCAGCTTGTCGCCCAA