| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042424 |
| Chromosome: | chromosome 5 |
| Location: | 3171250 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232000 | (1 of 1) IPR001623//IPR011989 - DnaJ domain // Armadillo-like helical | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGAAAAGGTACAGCACAGGCACGTGCACGTGAGCGTGAGCGTGAACG |
| Internal bar code: | CGTGTAGGGTTATAAGCAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1474 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCTCTTCACGTCGGTCC |
| Suggested primer 2: | AGGGGAGGAACACATGCATG |