Insertion junction: CLIP2.042433_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042433
Chromosome:chromosome 2
Location:5833819
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g117250 (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase 3'UTR
Cre02.g800244 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGATGCCGGCGGCACCTCGTATCGCGTATCTATCTAGCGCTGGCATTT
Internal bar code:AAAGCAAAGCGGTATAGACCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1681
LEAP-Seq percent confirming:52.381
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGACCTTCGCAGGGAGAT
Suggested primer 2:CTGTGCGATGTGCTATGTGC