| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042454 |
| Chromosome: | chromosome 14 |
| Location: | 4002409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAAGGCATGAAACGCGCAGCGGAGTGCATGTCGTAATTTGCTTGTACC |
| Internal bar code: | GATCTGGAAGTTCAGACAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3204 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGCTGTCCGTTGTGTTG |
| Suggested primer 2: | GAGGAGTCACCCAGGGTTTG |