Insertion junction: CLIP2.042459_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042459
Chromosome:chromosome 11
Location:4343486
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g482700 (1 of 3) PF09011 - HMG-box domain (HMG_box_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATATTCTACCGAACGACCTAGCTCGTGACAATGGTCACTTGCGGCACG
Internal bar code:ATCGAATTAATAGGAATACTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:97
LEAP-Seq percent confirming:8.33333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCTCACACGCACACTCAC
Suggested primer 2:CCTTTGTCCTTTGCGCTTCC