Insertion junction: CLIP2.042459_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.042459
Chromosome:chromosome 11
Location:4343491
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g482700 (1 of 3) PF09011 - HMG-box domain (HMG_box_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTAAAGCATGACGCGTATACGCTCCGCTTCGCAGCGGAACTGAAATAT
Internal bar code:AGCGCCTAGTCATTTGCTTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1294
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTTGTCCTTTGCGCTTCC
Suggested primer 2:TCTCTCACACGCACACTCAC