| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042461 |
| Chromosome: | chromosome 3 |
| Location: | 5377568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183451 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCGTGAAAACTGCGTTGCGCAATACCTTCAGACAGCCGAACGGGCCA |
| Internal bar code: | CGGGCCCTATATAGGTTCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2504 |
| LEAP-Seq percent confirming: | 75.6098 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTTCTCCCTTCCGAAA |
| Suggested primer 2: | CTGTGGTGTTGCATGTGTGG |