Insertion junction: CLIP2.042475_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042475
Chromosome:chromosome 2
Location:5032914
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g111000 RRA1,CGL9 (1 of 3) PTHR10994//PTHR10994:SF3 - RETICULON // ARABINOSYLTRANSFERASE; Reduced residual arabinose 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGGTCCTCGGACCCAAATTTGGTGTCCTGCACTGCATTTGCGCCTGT
Internal bar code:TCAGGTTTCTCGGGGAGTTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2274
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGCATGTCGATTGAGTTC
Suggested primer 2:GTACTGAGTGATAGCGCCCC