Insertion junction: CLIP2.042485_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042485
Chromosome:chromosome 13
Location:2792808
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g582650 MAPK1,LF4 (1 of 1) K08830 - renal tumor antigen [EC:2.7.11.22] (RAGE, MOK); Mitogen-activated protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCCTCCAGGCTGGCTCTGGTGTTTGAGCTCATGGACATGAACATCTA
Internal bar code:GTGTTTGAAGAGAGGCAATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:123
LEAP-Seq percent confirming:2.35294
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:83
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCTTGATGTCTCTGTGT
Suggested primer 2:GATCACACGCACGCACATAC