| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042486 |
| Chromosome: | chromosome 14 |
| Location: | 1440870 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617200 | RLS7 | RegA/Rls-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAACCTGCATGCGATCATGCAAGCGGTAATGTTGGCGAGCTCATGCG |
| Internal bar code: | TCGATTGATTCAGTTCACTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5061 |
| LEAP-Seq percent confirming: | 93.6937 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCAGACCCGACAGCATC |
| Suggested primer 2: | ACGAGTGCGGAAGTTGGATT |