| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042486 |
| Chromosome: | chromosome 14 |
| Location: | 1440885 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617200 | RLS7 | RegA/Rls-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCAGGTTTGAGAAGCTGGCCGGCTCCGCCACCGCCAAGTGGCACGT |
| Internal bar code: | ATAATCGCAGTCCTGACAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5532 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGTGCGGAAGTTGGATT |
| Suggested primer 2: | TATGCAGACCCGACAGCATC |