Insertion junction: CLIP2.042506_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042506
Chromosome:chromosome 6
Location:5954151
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290100 SYP6 Early endosomal Qc-SNARE protein, Syx6/Tlg1/Syntaxin 6-family (Qc.III.b); (1 of 1) K08498 - syntaxin 6 (STX6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGTGGGATGGGACACGCTGTACACTCCCACGCTGCAAACGCTGCACA
Internal bar code:CCAGCTTGGTTGACTTCCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1773
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TACAATCGTTGCAGCCCCAT
Suggested primer 2:GAGTCTCCCTCGCTATGCAC