Insertion junction: CLIP2.042531_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042531
Chromosome:chromosome 12
Location:3819906
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g514800 (1 of 2) K06287 - septum formation protein (maf) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCACACCACCTCCTCCATGCGACACATGACAGCACATGCACGCACATT
Internal bar code:TTTACTGGGCCGCTGCTGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:107
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGAACACCTCGCCCTCCT
Suggested primer 2:CCATGCAACACACAGGTTGG