| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042531 |
| Chromosome: | chromosome 12 |
| Location: | 3819910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514800 | (1 of 2) K06287 - septum formation protein (maf) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGTGAATGCGCGAGCCAATATAAGCATGTGTTTGAACTCACTGGAC |
| Internal bar code: | TTTACTGGGCCGCTGCTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2918 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCAACACACAGGTTGG |
| Suggested primer 2: | ATAGAACACCTCGCCCTCCT |