| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042538 |
| Chromosome: | chromosome 12 |
| Location: | 8729971 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547450 | VPS9 | Guanine nucleotide exchange factor, vacuolar; (1 of 1) PF02204 - Vacuolar sorting protein 9 (VPS9) domain (VPS9) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCTACCTCGGCCGTGCGCCTTCCCCCTGCCTTCCTCCCTGCTACTGC |
| Internal bar code: | TGACGGTCCCCTCGGAAACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGGAAGCTGCCAAGGAC |
| Suggested primer 2: | AAAACGGCTTCAACCCTCCA |