| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042546 |
| Chromosome: | chromosome 6 |
| Location: | 3325174 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277650 | BES4 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAAGCCGTTTCCGCCATCCTCCGCAGGCGGCGCCGGCTCGCTGGCGCC |
| Internal bar code: | ACTTGTAGGTAGGTGGTATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1805 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGGGCATTGACGAGAT |
| Suggested primer 2: | GCTACCGCCAGTATCCACTC |