Insertion junction: CLIP2.042547_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042547
Chromosome:chromosome 6
Location:3057534
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g274950 (1 of 1) K18953 - factor associated with neutral sphingomyelinase activation (NSMAF, FAN) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCTCACCCAGGTGTGGAGTAGTGTGTGCCGTACAGGAAGGGCGGCA
Internal bar code:CCTGGTTCAAGGCGATGGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGGCTGTTATGTGCGG
Suggested primer 2:GTTGAGGCATGGAGTCCACA