| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042551 |
| Chromosome: | chromosome 6 |
| Location: | 2565295 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269601 | (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGATGTTGAGTCACCCGCACCGGTCACGGCCATGGTGTTGGTCGAGTG |
| Internal bar code: | ACTGAAGGGGGAGGTGGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 54.7619 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACTGTCAAACCGCACAC |
| Suggested primer 2: | GTGTGCGGTTTGACAGTGAC |