Insertion junction: CLIP2.042566_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042566
Chromosome:chromosome 7
Location:2654648
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g330600 (1 of 2) K01510 - apyrase (ENTPD1_3_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATACATACTAAGGTCCCAGTGTGTCTCCATTGCCACACACACGCCACG
Internal bar code:GTTCTAGTGTTTAGAGCGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:727
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCGTGACAAAACTGCAAG
Suggested primer 2:ACCTCCTCTCGTCCTTCTCC