Insertion junction: CLIP2.042596_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.042596
Chromosome:chromosome 2
Location:1223246
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g081750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATTCCTGCTCTTCCGCAGCACGACGGTCGTGATCCTATCCATCGCGGT
Internal bar code:AAGCAGTAGCAAGTGGACCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1235
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTTTGCTGCCTTCTGTC
Suggested primer 2:CGACAGGTGTTGGTTTGCTG