| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042602 |
| Chromosome: | chromosome 10 |
| Location: | 6090052 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462050 | PTK20 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGCCGCGGAGGCCAGCCACGCGGACTGCGCGGCGTTGATGTCCGCGA |
| Internal bar code: | TTAGCATAAGCCTACGTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3084 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCTTGACGGTTCGTGTC |
| Suggested primer 2: | CCTGGCTACGTACGTCCATC |