| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042626 |
| Chromosome: | chromosome 12 |
| Location: | 2194256 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509150 | (1 of 1) PTHR18937//PTHR18937:SF151 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCTTGAGACTAAGCTGATCCGGGTCCGGGCCTGAACATAAGGGCACG |
| Internal bar code: | ACCCCTCCGTTCTGAAAGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5385 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCACCATTCCATGCACA |
| Suggested primer 2: | AGTTGGACATCAGCGCAAGA |