Insertion junction: CLIP2.042634_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042634
Chromosome:chromosome 11
Location:1293420
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467701 intron
Cre11.g467702 MRPS3,uS3m (1 of 3) PF00189 - Ribosomal protein S3, C-terminal domain (Ribosomal_S3_C); Mitochondrial ribosomal protein S3 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCAGACCAGGCCGGACCAGACCACCCACCAGCTCCAGCAGTCGGTCC
Internal bar code:CTTTCGGTTATGAAGCATTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:868
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTCTCCACATCCCCCAC
Suggested primer 2:GTACGGCAGGTACGAGGATG