| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.042635 |
| Chromosome: | chromosome 7 |
| Location: | 406422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314866 | EBM4 | (1 of 9) 3.2.1.78 - Mannan endo-1,4-beta-mannosidase / Endo-1,4-mannanase; Mannan endo-1%252C4-beta-mannosidase | intron |
| Cre07.g314900 | HEL34 | (1 of 1) PTHR24031:SF301 - ATP-DEPENDENT RNA HELICASE DDX18; DEAD/DEAH box helicase | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCGTCTGTCCCCGCGCACTCGGGCGCTATTGCATCGAGCAAACGCA |
| Internal bar code: | TACAGACTTGGACACCAAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGCATTCCTAGTCGTG |
| Suggested primer 2: | TGGGGAGCAGTTAACGGAAC |