| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042645 |
| Chromosome: | chromosome 12 |
| Location: | 9090099 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544401 | (1 of 1) PTHR20934:SF0 - TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGTCAGGGCCCTGGCGGGCGGATGAGATGGAGGTGAGGGACGAGGTC |
| Internal bar code: | GTACTCTAGATGTCCGTTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGCAACATTTTTGGCGA |
| Suggested primer 2: | AGCGAATGAGGCTTGGAGAC |