Insertion junction: CLIP2.042646_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042646
Chromosome:chromosome 7
Location:4603415
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344260 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCCCAGGTCCACCCAGCTAGACCAGACGCATTCCTCCGCACACCCCA
Internal bar code:CGTGCGCCTTGCGAGAAGAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1652
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGAAGTTTGTGCGAGCG
Suggested primer 2:ACTAACAGGCATGGTGGACG